Which of the statements given is incorrect?

Biology Biotechnology Principles and Processes Principles of Biotechnology and Restriction Enzymes Single Correct MCQ

Published on: August 13, 2026

In microinjection method, foreign DNA is directly injected into the nucleus of animal cell by using microneedles

Microinjection method is used in oocytes, eggs and embryo

Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride

In chemical mediated gene transfer method, certain chemicals such as Ca phosphate help foreign DNA to enter the host cell

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The correct answer is:

Electroporation is a physical method of gene transfer where short high-voltage electric pulses are applied to cells. This creates temporary pores in the plasma membrane, allowing foreign DNA to enter.

The statement says electroporation uses lysozyme or calcium chloride, which is incorrect. Those chemicals are used in chemical-mediated gene transfer, not electroporation.

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